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Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies

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Osteopathic Manipulative Treatment

BMC Res Notes. 2020 Aug 27;13(1):400.

Authors:

Aric Anloague, Aaron Mahoney, Oladipupo Ogunbekun, Taylor A Hiland, William R Thompson, Bryan Larsen, M Terry Loghmani, Julia M Hum, Jonathan W Lowery

Abstract.



Objective: Soft tissue manual therapies are commonly utilized by osteopathic physicians, chiropractors, physical therapists and massage therapists. These techniques are predicated on subjecting tissues to biophysical mechanical stimulation but the cellular and molecular mechanism(s) mediating these effects are poorly understood. Previous studies established an in vitro model system for examining mechanical stimulation of dermal fibroblasts and established that cyclical strain, intended to mimic overuse injury, induces secretion of numerous pro-inflammatory cytokines. Moreover, mechanical strain intended to mimic soft tissue manual therapy reduces strain-induced secretion of pro-inflammatory cytokines. Here, we sought to partially confirm and extend these reports and provide independent corroboration of prior results.

Results: Using cultures of primary human dermal fibroblasts, we confirm cyclical mechanical strain increases levels of IL-6 and adding long-duration stretch, intended to mimic therapeutic soft tissue stimulation, after cyclical strain results in lower IL-6 levels. We also extend the prior work, reporting that long-duration stretch results in lower levels of IL-8. Although there are important limitations to this experimental model, these findings provide supportive evidence that therapeutic soft tissue stimulation may reduce levels of pro-inflammatory cytokines. Future work is required to address these open questions and advance the mechanistic understanding of therapeutic soft tissue stimulation.

Publication Date: 

2020 Aug

OEID: 

3288

Anloague, A., Mahoney, A., Ogunbekun, O., Hiland, AT., Thompson, RW., Larsen, B., Loghmani, TM., Hum, MJ., Lowery, WJ. (2020) 'Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies ', BMC Res Notes. 2020 Aug 27;13(1):400.

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