Objective: To investigate the three-dimensional anatomical position of the rotatory center in cervical rotatory and local manipulation. Methods: A three-dimensional model was constructed based on CT data from cadaveric specimens of axial spinous process deviation. Cervical rotation was simulated on this model, and the graphic outcome was analyzed. Results: The rotatory center of this model was at the dens axis, not at the apex of the axial spionus process, and the angle of rotation exceeded observable angle. Conclusion: The new concept of anisotropic rotation of the cervical vertebrae should be established and its principles must be comprehended in clinical practice of cervical rotatory manipulation.